Temperature-dependent phase-separation, clouding, has been observed in suspensions of silica nanoparticles surface-functionalized with methyl-poly ethylene glycol silane. Interparticle interactions and conformational changes of the grafted poly ethylene glycol chains influence the observed cloud points, and can be controlled by electrolyte concentration and pH. These findings open new routes to tailoring properties of Pickering emulsions. The article was received on 15 Jan , accepted on 29 Mar and first published on 30 Apr Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.
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Pre-existing and induced anti-poly ethylene glycol PEG antibodies abs have been shown to be related with limitation of therapeutic efficacy and reduction in tolerance of several therapeutic agents. However, the current methods to detect anti-PEG abs are tedious and usually lack of quantification. A facile, rapid, sensitive and reliable technique to detect anti-PEG abs is highly desired in both research and clinic settings. The detection of anti-PEG abs directly from diluted blood serum was achieved within 40 min. Furthermore, different antibody isotypes can be quantitatively differentiated by adopting secondary antibodies.